Half life play time2/25/2023 Although direct measurements of mRNA decay rate can be performed with kinetic labeling techniques and transcriptional inhibitors, these techniques often introduce significant changes in cell physiology. Frequently, mRNA stability is studied indirectly by analyzing the steady-state level of mRNA in the cytoplasm in this case, changes in mRNA abundance are assumed to reflect only mRNA degradation, an assumption that is not always correct. The recognition of the importance of mRNA turnover in regulating eukaryotic gene expression has mandated the development of reliable, rigorous, and “user-friendly” methods to accurately measure changes in mRNA stability in mammalian cells.
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